An enhanced CRISPR repressor for targeted mammalian gene regulation.
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ABSTRACT:
SUBMITTER: Yeo NC
PROVIDER: S-EPMC6129399 | biostudies-literature | 2018 Aug
REPOSITORIES: biostudies-literature
Publications
An enhanced CRISPR repressor for targeted mammalian gene regulation.
Yeo Nan Cher NC Chavez Alejandro A Lance-Byrne Alissa A Chan Yingleong Y Menn David D Milanova Denitsa D Kuo Chih-Chung CC Guo Xiaoge X Sharma Sumana S Tung Angela A Cecchi Ryan J RJ Tuttle Marcelle M Pradhan Swechchha S Lim Elaine T ET Davidsohn Noah N Ebrahimkhani Mo R MR Collins James J JJ Lewis Nathan E NE Kiani Samira S Church George M GM Church George M GM
Nature methods 20180716 8
The RNA-guided endonuclease Cas9 can be converted into a programmable transcriptional repressor, but inefficiencies in target-gene silencing have limited its utility. Here we describe an improved Cas9 repressor based on the C-terminal fusion of a rationally designed bipartite repressor domain, KRAB-MeCP2, to nuclease-dead Cas9. We demonstrate the system's superiority in silencing coding and noncoding genes, simultaneously repressing a series of target genes, improving the results of single and d ...[more]
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