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Structure of the herpes simplex virus type 2 C-capsid with capsid-vertex-specific component.


ABSTRACT: Herpes simplex viruses (HSVs) cause human oral and genital ulcer diseases. Patients with HSV-2 have a higher risk of acquiring a human immunodeficiency virus infection. HSV-2 is a member of the ?-herpesvirinae subfamily that together with the ?- and ?-herpesvirinae subfamilies forms the Herpesviridae family. Here, we report the cryo-electron microscopy structure of the HSV-2 C-capsid with capsid-vertex-specific component (CVSC) that was determined at 3.75?Å using a block-based reconstruction strategy. We present atomic models of multiple conformers for the capsid proteins (VP5, VP23, VP19C, and VP26) and CVSC. Comparison of the HSV-2 homologs yields information about structural similarities and differences between the three herpesviruses sub-families and we identify ?-herpesvirus-specific structural features. The hetero-pentameric CVSC, consisting of a UL17 monomer, a UL25 dimer and a UL36 dimer, is bound tightly by a five-helix bundle that forms extensive networks of subunit contacts with surrounding capsid proteins, which reinforce capsid stability.

SUBMITTER: Wang J 

PROVIDER: S-EPMC6131487 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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Structure of the herpes simplex virus type 2 C-capsid with capsid-vertex-specific component.

Wang Jialing J   Yuan Shuai S   Zhu Dongjie D   Tang Hao H   Wang Nan N   Chen Wenyuan W   Gao Qiang Q   Li Yuhua Y   Wang Junzhi J   Liu Hongrong H   Zhang Xinzheng X   Rao Zihe Z   Wang Xiangxi X  

Nature communications 20180910 1


Herpes simplex viruses (HSVs) cause human oral and genital ulcer diseases. Patients with HSV-2 have a higher risk of acquiring a human immunodeficiency virus infection. HSV-2 is a member of the α-herpesvirinae subfamily that together with the β- and γ-herpesvirinae subfamilies forms the Herpesviridae family. Here, we report the cryo-electron microscopy structure of the HSV-2 C-capsid with capsid-vertex-specific component (CVSC) that was determined at 3.75 Å using a block-based reconstruction str  ...[more]

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