Ontology highlight
ABSTRACT:
SUBMITTER: Schroter C
PROVIDER: S-EPMC6132741 | biostudies-literature | 2018 Oct
REPOSITORIES: biostudies-literature
Schröter Christian C Beck Jan J Krah Simon S Zielonka Stefan S Doerner Achim A Rhiel Laura L Günther Ralf R Toleikis Lars L Kolmar Harald H Hock Björn B Becker Stefan S
Molecular biotechnology 20181001 10
In this study, we present a multiparameter screening procedure for the identification of target-specific antibodies with prescribed properties. Based on B cell receptor gene repertoires from transgenic rats, yeast surface display libraries were generated, and high-affinity human antibodies were readily isolated. We demonstrate that specific desirable features, i.e., species' cross-reactivity and a broad epitope coverage can be integrated into the screening procedure using high-throughput fluores ...[more]