Dataset Information

GWAS for Interleukin-1? levels in gingival crevicular fluid identifies IL37 variants in periodontal inflammation.

ABSTRACT: There is no agnostic GWAS evidence for the genetic control of IL-1? expression in periodontal disease. Here we report a GWAS for "high" gingival crevicular fluid IL-1? expression among 4910 European-American adults and identify association signals in the IL37 locus. rs3811046 at this locus (p?=?3.3?×?10-22) is associated with severe chronic periodontitis (OR?=?1.50; 95% CI?=?1.12-2.00), 10-year incident tooth loss (?3 teeth: RR?=?1.33; 95% CI?=?1.09-1.62) and aggressive periodontitis (OR?=?1.12; 95% CI?=?1.01-1.26) in an independent sample of 4927 German/Dutch adults. The minor allele at rs3811046 is associated with increased expression of IL-1? in periodontal tissue. In RAW macrophages, PBMCs and transgenic mice, the IL37 variant increases expression of IL-1? and IL-6, inducing more severe periodontal disease, while IL-37 protein production is impaired and shows reduced cleavage by caspase-1. A second variant in the IL37 locus (rs2708943, p?=?4.2?×?10-7) associates with attenuated IL37 mRNA expression. Overall, we demonstrate that IL37 variants modulate the inflammatory cascade in periodontal disease.

SUBMITTER: Offenbacher S

PROVIDER: S-EPMC6134146 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

GWAS for Interleukin-1β levels in gingival crevicular fluid identifies IL37 variants in periodontal inflammation.

Offenbacher Steven S Jiao Yizu Y Kim Steven J SJ Marchesan Julie J Moss Kevin L KL Jing Li L Divaris Kimon K Bencharit Sompop S Agler Cary S CS Morelli Thiago T Zhang Shaoping S Sun Lu L Seaman William T WT Cowley Dale D Barros Silvana P SP Beck James D JD Munz Matthias M Schaefer Arne S AS North Kari E KE

Nature communications 20180911 1

There is no agnostic GWAS evidence for the genetic control of IL-1β expression in periodontal disease. Here we report a GWAS for "high" gingival crevicular fluid IL-1β expression among 4910 European-American adults and identify association signals in the IL37 locus. rs3811046 at this locus (p = 3.3 × 10<sup>-22</sup>) is associated with severe chronic periodontitis (OR = 1.50; 95% CI = 1.12-2.00), 10-year incident tooth loss (≥3 teeth: RR = 1.33; 95% CI = 1.09-1.62) and aggressive periodontitis ...[more]

PMID: 30206230

Similar Datasets

Project description:Understanding the pathophysiology of the coronavirus disease 2019 (COVID-19) infection remains a significant challenge of our times. The gingival crevicular fluid being representative of systemic status and having a proven track record of detecting viruses and biomarkers forms a logical basis for evaluating the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The study aimed to assess gingival crevicular fluid (GCF) for evidence of SARS-CoV-2 in 33 patients who were deemed to be COVID-19 positive upon nasopharyngeal sampling. An attempt was also made to comparatively evaluate it with saliva in terms of its sensitivity, as a diagnostic fluid for SARS-CoV-2. GCF and saliva samples were collected from 33 COVID-19-confirmed patients. Total RNA was extracted using NucliSENS easyMAG (bioMérieux) and eluted in the elution buffer. Envelope gene (E gene) of SARS-CoV-2 and human RNase P gene as internal control were detected in GCF samples by using the TRUPCR SARS-CoV-2 RT qPCR kit V-2.0 (I) in an Applied Biosystems 7500 real-time machine. A significant majority of both asymptomatic and mildly symptomatic patients exhibited the presence of the novel coronavirus in their GCF samples. Considering the presence of SARS-CoV-2 RNA in the nasopharyngeal swab sampling as gold standard, the sensitivity of GCF and saliva, respectively, was 63.64% (confidence interval [CI], 45.1% to 79.60%) and 64.52% (CI, 45.37% to 80.77%). GCF was found to be comparable to saliva in terms of its sensitivity to detect SARS-CoV-2. Saliva samples tested positive in 3 of the 12 patients whose GCF tested negative, and likewise GCF tested positive for 2 of the 11 patients whose saliva tested negative on real-time reverse transcription polymerase chain reaction. The results establish GCF as a possible mode of transmission of SARS-CoV-2, which is the first such report in the literature, and also provide the first quantifiable evidence pointing toward a link between the COVID-19 infection and oral health.

Dataset Information

GWAS for Interleukin-1? levels in gingival crevicular fluid identifies IL37 variants in periodontal inflammation.

Publications

GWAS for Interleukin-1β levels in gingival crevicular fluid identifies IL37 variants in periodontal inflammation.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets