In vivo detection of optically-evoked opioid peptide release.
Ontology highlight
ABSTRACT:
SUBMITTER: Al-Hasani R
PROVIDER: S-EPMC6135606 | biostudies-literature | 2018 Sep
REPOSITORIES: biostudies-literature
Publications
In vivo detection of optically-evoked opioid peptide release.
Al-Hasani Ream R Wong Jenny-Marie T JT Mabrouk Omar S OS McCall Jordan G JG Schmitz Gavin P GP Porter-Stransky Kirsten A KA Aragona Brandon J BJ Kennedy Robert T RT Bruchas Michael R MR
eLife 20180903
Though the last decade has seen accelerated advances in techniques and technologies to perturb neuronal circuitry in the brain, we are still poorly equipped to adequately dissect endogenous peptide release in vivo. To this end we developed a system that combines in vivo optogenetics with microdialysis and a highly sensitive mass spectrometry-based assay to measure opioid peptide release in freely moving rodents. ...[more]
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