Ontology highlight
ABSTRACT:
SUBMITTER: Selyutina A
PROVIDER: S-EPMC6135678 | biostudies-literature | 2018 Oct
REPOSITORIES: biostudies-literature
Selyutina Anastasia A Bulnes-Ramos Angel A Diaz-Griffero Felipe F
Virology 20180726
The capsid-binding assay is an in vitro experiment used to determine whether cellular proteins interact with the HIV-1 core. In vitro assembled HIV-1 capsids recapitulate the surface of the HIV-1 core. The assay involves the incubation of in vitro assembled HIV-1 capsid-nucleocapsid (CA-NC) complexes with the protein in question. Subsequently, the mixture is spun through a sucrose cushion using an ultracentrifuge, and the pellet is analyzed for the presence of the protein in question. Although t ...[more]