Ontology highlight
ABSTRACT:
SUBMITTER: Balintova J
PROVIDER: S-EPMC6137436 | biostudies-literature | 2018 Sep
REPOSITORIES: biostudies-literature
Balintová J J Welter M M Marx A A
Chemical science 20180724 35
Here we report on the development of an antibody-modified nucleotide and its sequence-selective incorporation into nascent DNA catalysed by DNA polymerases. Although the modification of the nucleotide is several orders of magnitude larger than the natural dNTP substrate and even exceeds the size of the DNA polymerase, it is well accepted by the enzyme. Moreover, the recognition of the antibody is not abolished by the conjugation but can be recognized by a secondary antibody that is conjugated to ...[more]