Ontology highlight
ABSTRACT:
SUBMITTER: Lin YC
PROVIDER: S-EPMC6138433 | biostudies-literature | 2018 Sep
REPOSITORIES: biostudies-literature
Lin Ying-Cing YC Pecetta Simone S Steichen Jon M JM Kratochvil Sven S Melzi Eleonora E Arnold Johan J Dougan Stephanie K SK Wu Lin L Kirsch Kathrin H KH Nair Usha U Schief William R WR Batista Facundo D FD
The EMBO journal 20180807 18
Here, we describe a one-step, <i>in vivo</i> CRISPR/Cas9 nuclease-mediated strategy to generate knock-in mice. We produced knock-in (KI) mice wherein a 1.9-kb DNA fragment bearing a pre-arranged human B-cell receptor heavy chain was recombined into the native murine immunoglobulin locus. Our methodology relies on Cas9 nuclease-induced double-stranded breaks directed by two sgRNAs to occur within the specific target locus of fertilized oocytes. These double-stranded breaks are subsequently repair ...[more]