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Synthesis of CID-cleavable protein crosslinking agents containing quaternary amines for structural mass spectrometry.

ABSTRACT: Two novel cyclic quaternary amine crosslinking probes are synthesized for structural mass spectrometry of protein complexes in solution and for analysis of protein interactions in organellar and whole cell extracts. Each exhibits high aqueous solubility, excellent protein crosslinking efficiencies, low collision induced dissociation (CID) energy fragmentation efficiencies, high stoichiometries of reaction, increased charges of crosslinked peptide ions, and maintenance of overall surface charge balance of crosslinked proteins.

SUBMITTER: Hagen SE

PROVIDER: S-EPMC6138586 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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Synthesis of CID-cleavable protein crosslinking agents containing quaternary amines for structural mass spectrometry.

Hagen Susan E SE Liu Kun K Jin Yafei Y Piersimoni Lolita L Andrews Philip C PC Showalter Hollis D HD

Organic & biomolecular chemistry 20180901 37

Two novel cyclic quaternary amine crosslinking probes are synthesized for structural mass spectrometry of protein complexes in solution and for analysis of protein interactions in organellar and whole cell extracts. Each exhibits high aqueous solubility, excellent protein crosslinking efficiencies, low collision induced dissociation (CID) energy fragmentation efficiencies, high stoichiometries of reaction, increased charges of crosslinked peptide ions, and maintenance of overall surface charge b ...[more]

PMID: 29537042

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