Ontology highlight
ABSTRACT:
SUBMITTER: Yang JS
PROVIDER: S-EPMC6138660 | biostudies-literature | 2018 Sep
REPOSITORIES: biostudies-literature
Nature communications 20180914 1
Knowing which proteins and RNAs directly interact is essential for understanding cellular mechanisms. Unfortunately, discovering such interactions is costly and often unreliable. To overcome these limitations, we developed rec-YnH, a new yeast two and three-hybrid-based screening pipeline capable of detecting interactions within protein libraries or between protein libraries and RNA fragment pools. rec-YnH combines batch cloning and transformation with intracellular homologous recombination to g ...[more]