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Simultaneous detection of many T-cell specificities using combinatorial tetramer staining.

ABSTRACT: The direct detection of antigen-specific T cells using tetramers of soluble peptide-major histocompatibilty complex (pMHC) molecules is widely used in both basic and clinical immunology. However, the number of specificities that can be assessed simultaneously has been a major limitation. Here we describe and validate a method using combinations of fluorescent pMHC tetramers to simultaneously detect and enrich for many (>or=15) T-cell specificities in a single human blood sample.

SUBMITTER: Newell EW

PROVIDER: S-EPMC2731062 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature

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Simultaneous detection of many T-cell specificities using combinatorial tetramer staining.

Newell Evan W EW Klein Lawrence O LO Yu Wong W Davis Mark M MM

Nature methods 20090621 7

The direct detection of antigen-specific T cells using tetramers of soluble peptide-major histocompatibilty complex (pMHC) molecules is widely used in both basic and clinical immunology. However, the number of specificities that can be assessed simultaneously has been a major limitation. Here we describe and validate a method using combinations of fluorescent pMHC tetramers to simultaneously detect and enrich for many (>or=15) T-cell specificities in a single human blood sample. ...[more]

PMID: 19543286

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