Ontology highlight
ABSTRACT:
SUBMITTER: Newell EW
PROVIDER: S-EPMC2731062 | biostudies-literature | 2009 Jul
REPOSITORIES: biostudies-literature
Newell Evan W EW Klein Lawrence O LO Yu Wong W Davis Mark M MM
Nature methods 20090621 7
The direct detection of antigen-specific T cells using tetramers of soluble peptide-major histocompatibilty complex (pMHC) molecules is widely used in both basic and clinical immunology. However, the number of specificities that can be assessed simultaneously has been a major limitation. Here we describe and validate a method using combinations of fluorescent pMHC tetramers to simultaneously detect and enrich for many (>or=15) T-cell specificities in a single human blood sample. ...[more]