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Data on a thermostable enzymatic one-pot reaction for the production of a high-value compound from l-arabinose.

ABSTRACT: The dataset presented in this article is related to the research article entitled "One-pot, two-step transaminase and transketolase synthesis of l-gluco-heptulose from l-arabinose" (Bawn et al., 2018 in press) [1]. This article presents data on initial experiments that were carried out to investigate new thermostable transketolase (TK) activities with l-arabinose. Transaminase (TAm) sequences from an in-house library of thermophilic strains were analyzed to compare homologies to characterized TAms with desired activity. DNA and amino acid sequences are presented for all the enzymes investigated. Calibration curves for products of the TK and TAm reactions are also presented along with chromatographic analysis of the various one-pot reactions.

SUBMITTER: Bawn M

PROVIDER: S-EPMC6140825 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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Data on a thermostable enzymatic one-pot reaction for the production of a high-value compound from l-arabinose.

Bawn Maria M Subrizi Fabiana F Lye Gary J GJ Sheppard Tom D TD Hailes Helen C HC Ward John M JM

Data in brief 20180531

The dataset presented in this article is related to the research article entitled "One-pot, two-step transaminase and transketolase synthesis of l-<i>gluco</i>-heptulose from l-arabinose" (Bawn et al., 2018 in press) [1]. This article presents data on initial experiments that were carried out to investigate new thermostable transketolase (TK) activities with l-arabinose. Transaminase (TAm) sequences from an in-house library of thermophilic strains were analyzed to compare homologies to character ...[more]

PMID: 30229010

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