Dataset Information

Data on biosurfactant assisted removal of TNT from contaminated soil.

ABSTRACT: Contamination of environment, especially soil, is in great concern and can cause health problems. Thus, remediation of these pollutants through environmentally friendly methods should be considered. The aim of this data was bioremediation of TNT from contaminated soil. Two plastic pans were used as bioreactor. In each pan, 3?kg of soil was used. Concentration of TNT in contaminated soil was 1000?mg/kg. Rhamnolipid in concentration of 60?mg/l was added to intended pan. Sampling was done in each two weeks. In order to assessment of TNT degradation, samples were analyzed with HPLC. The data showed that after 154 days of experiment, TNT removal in soil that amended with rhamnolipid was 73% and in experiment with no addition of rhamnolipid was 58%. Based on the obtained data rhamnolipid was effective in remediation of TNT contaminated soil.

SUBMITTER: Bina B

PROVIDER: S-EPMC6141866 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Data on biosurfactant assisted removal of TNT from contaminated soil.

Bina Bijan B Amin Mohammad Mehdi MM Kamarehie Bahram B Jafari Ali A Ghaderpoori Mansour M Karami Mohammad Amin MA Teimouri Fahimeh F Sadani Mohsen M

Data in brief 20180622

Contamination of environment, especially soil, is in great concern and can cause health problems. Thus, remediation of these pollutants through environmentally friendly methods should be considered. The aim of this data was bioremediation of TNT from contaminated soil. Two plastic pans were used as bioreactor. In each pan, 3 kg of soil was used. Concentration of TNT in contaminated soil was 1000 mg/kg. Rhamnolipid in concentration of 60 mg/l was added to intended pan. Sampling was done in each t ...[more]

PMID: 30246077

Similar Datasets

Project description:Polychlorinated biphenyls (PCBs) are persistent organic pollutants widely distributed in the environment and possess deleterious health effects. The main objective of the study was to obtain bacterial isolates from PCB-contaminated soil for enhanced biodegradation of PCB-77. Selective enrichment resulted in the isolation of 33 strains of PCB-contaminated soil nearby Bhilai steel plant, Chhattisgarh, India. Based on the prominent growth using biphenyl as the sole carbon source and the confirmation of its degradation by GC-MS/MS analysis, four isolates were selected for further study. The isolates identified by 16S rRNA gene sequencing were Pseudomonas aeruginosa MAPB-2, Pseudomonas plecoglossicida MAPB-6, Brucella anthropi MAPB-9, and Priestia megaterium MAPB-27. The isolate MAPB-9 showed a degradation of 66.15% biphenyl, while MAPB-2, MAPB-6, and MAPB-27 showed a degradation of 62.06, 57.02, and 56.55%, respectively in 48 h. Additionally, the degradation ability of these strains was enhanced with addition of co-metabolite glucose (0.2%) in the culture medium. Addition of glucose showed 100% degradation of biphenyl by MAPB-9, in 48 h, while MAPB-6, MAPB-2, and MAPB-27 showed 97.1, 67.5, and 53.3% degradation, respectively as analyzed by GC-MS/MS. Furthermore, in the presence of inducer, PCB-77 was found to be 59.89, 30.49, 27.19, and 4.43% degraded by MAPB-6, MAPB-9, MAPB-2, and MAPB-27, respectively in 7 d. The production of biosurfactants that aid in biodegradation process were observed in all the isolates. This was confirmed by ATR-FTIR analysis that showed the presence of major functional groups (CH2, CH3, CH, = CH2, C-O-C, C-O) of the biosurfactant. The biosurfactants were further identified by HPTLC and GC-MS/MS analysis. Present study is the first to report PCB-77 degradation potential of Pseudomonas aeruginosa, B. anthropi, Pseudomonas plecoglossicida, and Priestia megaterium. Similarly, this is the first report on Pseudomonas plecoglossicida and Priestia megaterium for PCB biodegradation. Our results suggest that the above isolates can be used for the biodegradation of biphenyl and PCB-77 in PCB-contaminated soil.

Project description:Petroleum is, up to this date, an inimitable nonrenewable energy resource. Petroleum leakage, which arises during transport, storage, and refining, is the most important contaminant in the environment, as it produces harm to the surrounding ecosystem. Bioremediation is an efficient method used to treat petroleum hydrocarbon-contaminated soil using indigenous microorganisms. The degradation characteristics for a variety of hydrocarbons (hexane, benzene, gasoline, and diesel) were qualitatively and quantitatively investigated using Bacillus isolates. Microbiological and biochemical methods have been used including isolation of oil-degrading bacteria, enzymatic activities, the determination of physicochemical parameters, biosurfactant production and extraction assay, oil displacement assay, antimicrobial assay of the biosurfactants, and bioremediation kinetics. Consequently, of the 60 isolates capable of degrading different hydrocarbons at fast rates, 34 were suspected to be Bacillus isolates capable of growing in 24 h or 48 h on BH medium supplemented with 2% of hexane, benzene, gasoline, diesel, and olive oil, respectively. Among the 34 isolates, 61% (21/34) are capable of producing biosurfactant-like molecules by using gasoline, 70% (24/34) with diesel oil, 85% (29/34) with hexane, and 82% (28/34) with benzene. It was found that biosurfactant-producing isolates are extractable with HCl (100%), ammonium sulphate (95%), chloroform (95%), and ethanol (100%). Biosurfactants showed stability at 20°C, 37°C, 40°C, and 60°C. Biosurfactant secreted by Bacillus strains has shown an antagonistic effect in Escherichia coli, Shigella flexneri 5a M90T, and Bacillus cereus. The selected isolates could therefore be safely used for biodegradation. Substrate biodegradation patterns by individual isolates were found to significantly differ. The study shows that benzene was degraded faster, followed by hexane, gasoline, and finally diesel. The Bacillus consortium used can decrease hydrocarbon content from 195 to 112 (g/kg) in 15 days.

Dataset Information

Data on biosurfactant assisted removal of TNT from contaminated soil.

Publications

Data on biosurfactant assisted removal of TNT from contaminated soil.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets