Ontology highlight
ABSTRACT:
SUBMITTER: Taheri-Ghahfarokhi A
PROVIDER: S-EPMC6144780 | biostudies-literature | 2018 Sep
REPOSITORIES: biostudies-literature
Taheri-Ghahfarokhi Amir A Taylor Benjamin J M BJM Nitsch Roberto R Lundin Anders A Cavallo Anna-Lina AL Madeyski-Bengtson Katja K Karlsson Fredrik F Clausen Maryam M Hicks Ryan R Mayr Lorenz M LM Bohlooly-Y Mohammad M Maresca Marcello M
Nucleic acids research 20180901 16
The mutation patterns at Cas9 targeted sites contain unique information regarding the nuclease activity and repair mechanisms in mammalian cells. However, analytical framework for extracting such information are lacking. Here, we present a novel computational platform called Rational InDel Meta-Analysis (RIMA) that enables an in-depth comprehensive analysis of Cas9-induced genetic alterations, especially InDels mutations. RIMA can be used to quantitate the contribution of classical microhomology ...[more]