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Application of Ammonium Persulfate for Selective Oxidation of Guanines for Nucleic Acid Sequencing.

ABSTRACT: Nucleic acids can be sequenced by a chemical procedure that partially damages the nucleotide positions at their base repetition. Many methods have been reported for the selective recognition of guanine. The accurate identification of guanine in both single and double regions of DNA and RNA remains a challenging task. Herein, we present a new, non-toxic and simple method for the selective recognition of guanine in both DNA and RNA sequences via ammonium persulfate modification. This strategy can be further successfully applied to the detection of 5-methylcytosine by using PCR.

SUBMITTER: Wang Y

PROVIDER: S-EPMC6152272 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

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Application of Ammonium Persulfate for Selective Oxidation of Guanines for Nucleic Acid Sequencing.

Wang Yafen Y Liu Chaoxing C Hong Tingting T Wu Fan F Yu Shuyi S He Zhiyong Z Mao Wuxiang W Zhou Xiang X

Molecules (Basel, Switzerland) 20170721 7

Nucleic acids can be sequenced by a chemical procedure that partially damages the nucleotide positions at their base repetition. Many methods have been reported for the selective recognition of guanine. The accurate identification of guanine in both single and double regions of DNA and RNA remains a challenging task. Herein, we present a new, non-toxic and simple method for the selective recognition of guanine in both DNA and RNA sequences via ammonium persulfate modification. This strategy can ...[more]

PMID: 28753999

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