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Anti-Proliferative Effect of Triterpenoidal Glycosides from the Roots of Anemone vitifolia through a Pro-Apoptotic Way.

ABSTRACT: A cytotoxicity-guided phytochemical investigation of Anemone vitifolia roots led to the isolation of six oleanane saponins (1-6), which were reported from the species for the first time. Their structures were determined by comparing its MS and NMR data with those in literature. Compounds 1-4 showed significant inhibitory effects on the proliferation of hepatocellular carcinoma HepG2 cells with IC₅₀ values ranging from 2.0 to 8.5 ?M, compared to positive control methotrexate with IC₅₀ value of 15.8 ?M. Flow cytometry analysis revealed that compounds 1-4 exerted anti-proliferative effects through a pro-apoptotic way of hepatocellular carcinoma cells.

SUBMITTER: Bai C

PROVIDER: S-EPMC6154594 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature

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Anti-Proliferative Effect of Triterpenoidal Glycosides from the Roots of Anemone vitifolia through a Pro-Apoptotic Way.

Bai Changcai C Ye Yunyun Y Feng Xiao X Bai Ruifeng R Han Lu L Zhou Xiuping X Yang Xinyao X Tu Pengfei P Chai Xingyun X

Molecules (Basel, Switzerland) 20170417 4

A cytotoxicity-guided phytochemical investigation of Anemone vitifolia roots led to the isolation of six oleanane saponins (1-6), which were reported from the species for the first time. Their structures were determined by comparing its MS and NMR data with those in literature. Compounds 1-4 showed significant inhibitory effects on the proliferation of hepatocellular carcinoma HepG2 cells with IC50 values ranging from 2.0 to 8.5 μM, compared to posit ...[more]

PMID: 28420183

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