Individual islet respirometry reveals functional diversity within the islet population of mice and human donors.

ABSTRACT:

Objective

Islets from the same pancreas show remarkable variability in glucose sensitivity. While mitochondrial respiration is essential for glucose-stimulated insulin secretion, little is known regarding heterogeneity in mitochondrial function at the individual islet level. This is due in part to a lack of high-throughput and non-invasive methods for detecting single islet function.

Methods

We have developed a novel non-invasive, high-throughput methodology capable of assessing mitochondrial respiration in large-sized individual islets using the XF96 analyzer (Agilent Technologies).

Results

By increasing measurement sensitivity, we have reduced the minimal size of mouse and human islets needed to assess mitochondrial respiration to single large islets of >35,000 μm

SUBMITTER: Taddeo EP 

PROVIDER: S-EPMC6157638 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature