Ontology highlight
ABSTRACT:
SUBMITTER: Zhou KI
PROVIDER: S-EPMC6161689 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
RNA biology 20180511 7
The abundant RNA modification pseudouridine (Ψ) has been mapped transcriptome-wide by chemically modifying pseudouridines with carbodiimide and detecting the resulting reverse transcription stops in high-throughput sequencing. However, these methods have limited sensitivity and specificity, in part due to the use of reverse transcription stops. We sought to use mutations rather than just stops in sequencing data to identify pseudouridine sites. Here, we identify reverse transcription conditions ...[more]