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Detection of virus-specific polymeric immunoglobulin A in acute hepatitis A, C, E virus serum samples using novel chimeric secretory component.

ABSTRACT: OBJECTIVE:To conduct a proof-of-concept study on preferential binding of polymeric IgA (pIgA) using a novel recombinant rabbit/human chimeric secretory component (cSC) and preliminary assessment of the diagnostic potential of virus-specific pIgA in discriminating acute hepatitis A, E, and C (HAV, HEV, HCV) patients and uninfected controls using an indirect enzyme-linked immunoassay. RESULTS:cSC binds >?0.06 ?g/ml of purified human and mouse pIgA with negligible cross-reactivity against IgM and IgA. Virus-specific pIgA was significantly higher in serum of acute HAV (n?=?6) and HEV (n?=?12) patients than uninfected samples (HEV: p?

SUBMITTER: Mohd Hanafiah K

PROVIDER: S-EPMC6167832 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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Detection of virus-specific polymeric immunoglobulin A in acute hepatitis A, C, E virus serum samples using novel chimeric secretory component.

Mohd Hanafiah Khayriyyah K Garcia Mary L ML Barnes Nadine C NC Anderson David A DA

BMC research notes 20181001 1

<h4>Objective</h4>To conduct a proof-of-concept study on preferential binding of polymeric IgA (pIgA) using a novel recombinant rabbit/human chimeric secretory component (cSC) and preliminary assessment of the diagnostic potential of virus-specific pIgA in discriminating acute hepatitis A, E, and C (HAV, HEV, HCV) patients and uninfected controls using an indirect enzyme-linked immunoassay.<h4>Results</h4>cSC binds > 0.06 μg/ml of purified human and mouse pIgA with negligible cross-reactivity ag ...[more]

PMID: 30285838

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