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FGF2 Enhances Odontoblast Differentiation by ?SMA⁺ Progenitors In Vivo.

ABSTRACT: The goal of this study was to examine the effects of early and limited exposure of perivascular cells expressing ? (?SMA) to fibroblast growth factor 2 (FGF2) in vivo. We performed in vivo fate mapping by inducible Cre-loxP and experimental pulp injury in molars to induce reparative dentinogenesis. Our results demonstrate that early delivery of exogenous FGF2 to exposed pulp led to proliferative expansion of ?SMA-tdTomato⁺ cells and their accelerated differentiation into odontoblasts. In vivo lineage-tracing experiments showed that the calcified bridge/reparative dentin in FGF2-treated pulps were lined with an increased number of Dspp⁺ odontoblasts and devoid of BSP⁺ osteoblasts. The increased number of odontoblasts derived from ?SMA-tdTomato⁺ cells and the formation of reparative dentin devoid of osteoblasts provide in vivo evidence for the stimulatory effects of FGF signaling on odontoblast differentiation from early progenitors in dental pulp.

SUBMITTER: Vidovic-Zdrilic I

PROVIDER: S-EPMC6169028 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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FGF2 Enhances Odontoblast Differentiation by αSMA<sup>+</sup> Progenitors In Vivo.

Vidovic-Zdrilic I I Vining K H KH Vijaykumar A A Kalajzic I I Mooney D J DJ Mina M M

Journal of dental research 20180412 10

The goal of this study was to examine the effects of early and limited exposure of perivascular cells expressing α (αSMA) to fibroblast growth factor 2 (FGF2) in vivo. We performed in vivo fate mapping by inducible Cre-loxP and experimental pulp injury in molars to induce reparative dentinogenesis. Our results demonstrate that early delivery of exogenous FGF2 to exposed pulp led to proliferative expansion of αSMA-tdTomato<sup>+</sup> cells and their accelerated differentiation into odontoblasts. ...[more]

PMID: 29649366

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Project description:To assess the effect of fibronectin (Fn) and porcine type I collagen (PCOL) on odontoblast-like cells in vitro.Rat odontoblast-like cells (MDPC-23 cells) were inoculated and cultured on Fn-coated or type I collagen-coated substrates. Proliferation assay, alkaline phosphatase activity (ALP activity), mRNA expression of hard tissue-forming markers, and Alizarin red staining were investigated over a period of 10 days.Cells maintained a high proliferation activity on Fn and PCOL even at a low seeding concentration (0.5×104/mL) as demonstrated by CCK-8 assay. The proliferation activity of cells on Fn increases in a concentration-dependent manner while it reached a plateau after 10 µg/mL. Cells adopted long, thin and spindle shape on Fn(10-50) and PCOL. Parallel actin filaments were observed in MDPC-23 cells cultured on Fn and PCOL. ALP activity was markedly up-regulated on Fn and PCOL-coated surfaces. Importantly, gene expression of BSP (Fn10: 2.44±0.32; Fn20: 3.05±0.01; Fn30: 2.90±0.21; Fn40: 2.74±0.30; Fn50: 2.64±0.12; PCOL: 2.20±0.03) and OCN (Fn10: 2.52±0.23; Fn20: 2.28±0.24; Fn30: 2.34±0.21; Fn40: 2.34±0.25; Fn50: 2.20±0.22; PCOL: 1.56±0.16) was significantly enhanced on Fn and PCOL substrates as compared with control; moreover, expression of integrin beta 1 (ITGB1), an ubiquitous cell surface receptor was augmented in Fn(10-50) and PCOL groups simultaneously. In accordance with the ALP activity and gene expression data, calcific deposition in cells grown on Fn(10-50) and PCOL was observed as well.Despite the limitation of this study, the findings indicate that a surface coating of Fn enhances the proliferation, differentiation and mineralization of odontoblast-like cells by activation of integrin beta 1 (ITG B1). The promoting effects of Fn on MDPC-23 cells were achieved at a comparatively lower coating concentration than type I collagen (300 µg/mL). Specifically, it is suggested that the optimum coating concentration of Fn to be 10 µg/mL.

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FGF2 Enhances Odontoblast Differentiation by ?SMA⁺ Progenitors In Vivo.

Publications

FGF2 Enhances Odontoblast Differentiation by αSMA<sup>+</sup> Progenitors In Vivo.

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Dataset Information

FGF2 Enhances Odontoblast Differentiation by ?SMA+ Progenitors In Vivo.

Publications

FGF2 Enhances Odontoblast Differentiation by αSMA<sup>+</sup> Progenitors In Vivo.

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OmicsDI is part of the ELIXIR infrastructure

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FGF2 Enhances Odontoblast Differentiation by ?SMA⁺ Progenitors In Vivo.