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One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation.

ABSTRACT: We developed a method for rapid generation of B cell receptor (BCR) monoclonal mice expressing prerearranged Igh and Igk chains monoallelically from the Igh locus by CRISPR-Cas9 injection into fertilized oocytes. B cells from these mice undergo somatic hypermutation (SHM), class switch recombination (CSR), and affinity-based selection in germinal centers. This method combines the practicality of BCR transgenes with the ability to study Ig SHM, CSR, and affinity maturation.

SUBMITTER: Jacobsen JT

PROVIDER: S-EPMC6170169 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation.

Jacobsen Johanne T JT Mesin Luka L Markoulaki Styliani S Schiepers Ariën A Cavazzoni Cecília B CB Bousbaine Djenet D Jaenisch Rudolf R Victora Gabriel D GD

The Journal of experimental medicine 20180904 10

We developed a method for rapid generation of B cell receptor (BCR) monoclonal mice expressing prerearranged Igh and Igk chains monoallelically from the Igh locus by CRISPR-Cas9 injection into fertilized oocytes. B cells from these mice undergo somatic hypermutation (SHM), class switch recombination (CSR), and affinity-based selection in germinal centers. This method combines the practicality of BCR transgenes with the ability to study Ig SHM, CSR, and affinity maturation. ...[more]

PMID: 30181412

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