Ontology highlight
ABSTRACT:
SUBMITTER: Kaberniuk AA
PROVIDER: S-EPMC6170497 | biostudies-literature | 2018 Oct
REPOSITORIES: biostudies-literature
Kaberniuk Andrii A AA Mohr Manuel A MA Verkhusha Vladislav V VV Snapp Erik Lee EL
Scientific reports 20181003 1
The ability of fluorescent proteins (FPs) to fold robustly is fundamental to the autocatalytic formation of the chromophore. While the importance of the tertiary protein structure is well appreciated, the impact of individual amino acid mutations for FPs is often not intuitive and requires direct testing. In this study, we describe the engineering of a monomeric photoswitchable FP, moxMaple3, for use in oxidizing cellular environments, especially the eukaryotic secretory pathway. Surprisingly, a ...[more]