Ontology highlight
ABSTRACT:
SUBMITTER: Wang Y
PROVIDER: S-EPMC6174115 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Wang Yong Y Tian Kai K Du Xiao X Shi Rui-Cheng RC Gu Li-Qun LQ
Analytical chemistry 20171204 24
Aerolysin protein pore has been widely used for sensing peptides and proteins. However, only a few groups explored this nanopore for nucleic acids detection. The challenge is the extremely low capture efficiency for nucleic acids (>10 bases), which severely lowers the sensitivity of an aerolysin-based genetic biosensor. Here we reported a simple and easy-to-operate approach to noncovalently transform aerolysin into a highly nucleic acids-sensitive nanopore. Through a remote pH-modulation mechani ...[more]