Ontology highlight
ABSTRACT:
SUBMITTER: Sundarrajan S
PROVIDER: S-EPMC6175717 | biostudies-literature | 2018 Oct - Dec
REPOSITORIES: biostudies-literature
Sundarrajan Sudarson S Rao Sneha S Padmanabhan Sriram S
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] 20180412 4
We studied the role of Thermus thermophilus Recombinase A (RecA) in enhancing the PCR signals of DNA viruses such as Hepatitis B virus (HBV). The RecA gene of a thermophilic eubacterial strain, T. thermophilus, was cloned and hyperexpressed in Escherichia coli. The recombinant RecA protein was purified using a single heat treatment step without the use of any chromatography steps, and the purified protein (>95%) was found to be active. The purified RecA could enhance the polymerase chain reactio ...[more]