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Identification of cell populations necessary for leaf-to-leaf electrical signaling in a wounded plant.


ABSTRACT: The identity of the cell files necessary for the leaf-to-leaf transmission of wound signals plants has been debated for decades. In Arabidopsis, wounding initiates the glutamate receptor-like (GLR)-dependent propagation of membrane depolarizations that lead to defense gene activation. Using a vein extraction procedure we found pools of GLR-fusion proteins in endomembranes in phloem sieve elements and/or in xylem contact cells. Strikingly, only double mutants that eliminated GLRs from both of these spatially separated cell types strongly attenuated leaf-to-leaf electrical signaling. glr3.3 mutants were also compromised in their defense against herbivores. Since wounding is known to cause increases in cytosolic calcium, we monitored electrical signals and Ca2+ transients simultaneously. This revealed that wound-induced membrane depolarizations in the wild-type preceded cytosolic Ca2+ maxima. The axial and radial distributions of calcium fluxes were differentially affected in each glr mutant. Resolving a debate over which cell types are necessary for electrical signaling between leaves, we show that phloem sieve elements and xylem contact cells function together in this process.

SUBMITTER: Nguyen CT 

PROVIDER: S-EPMC6176584 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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Identification of cell populations necessary for leaf-to-leaf electrical signaling in a wounded plant.

Nguyen Chi Tam CT   Kurenda Andrzej A   Stolz Stéphanie S   Chételat Aurore A   Farmer Edward E EE  

Proceedings of the National Academy of Sciences of the United States of America 20180918 40


The identity of the cell files necessary for the leaf-to-leaf transmission of wound signals plants has been debated for decades. In <i>Arabidopsis</i>, wounding initiates the glutamate receptor-like (GLR)-dependent propagation of membrane depolarizations that lead to defense gene activation. Using a vein extraction procedure we found pools of GLR-fusion proteins in endomembranes in phloem sieve elements and/or in xylem contact cells. Strikingly, only double mutants that eliminated GLRs from both  ...[more]

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