The role of acetyltransferases for the temporal-specific accessibility of ?-catenin to the myogenic gene locus.

ABSTRACT: Molecules involved in WNT/?-catenin signaling show spatiotemporal-specific expression and play vital roles in muscle development. Our previous study showed that WNT/?-catenin signaling promotes myoblast proliferation and differentiation through the regulation of the cyclin A2 (Ccna2)/cell division cycle 25C (Cdc25c) and Fermitin family homolog 2 (Fermt2) genes, respectively. However, it remains unclear how ?-catenin targets different genes from stage to stage during myogenesis. Here, we show that the accessibility of ?-catenin to the promoter region of its target genes is regulated by developmental stage-specific histone acetyltransferases (HATs), lysine acetyltransferase 2B (KAT2B), and cAMP-response element-binding protein (CREB)-binding protein (CBP). We found that KAT2B was specifically expressed at the myoblast proliferation stage and formed a complex with ?-catenin to induce Ccna2/Cdc25c expression. On the other hand, CBP was specifically expressed during myoblast differentiation and formed a complex with ?-catenin to induce Fermt2 expression. Our findings indicate that ?-catenin efficiently accesses to its target gene's promoters by forming a complex with developmental stage-specific acetyltransferases during myogenesis.

SUBMITTER: Suzuki A 

PROVIDER: S-EPMC6180044 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature