Ontology highlight
ABSTRACT:
SUBMITTER: Bottermann M
PROVIDER: S-EPMC6187179 | biostudies-literature | 2018 Oct
REPOSITORIES: biostudies-literature
Bottermann Maria M Foss Stian S van Tienen Laurens M LM Vaysburd Marina M Cruickshank James J O'Connell Kevin K Clark Jessica J Mayes Keith K Higginson Katie K Hirst Jack C JC McAdam Martin B MB Slodkowicz Greg G Hutchinson Edward E Kozik Patrycja P Andersen Jan Terje JT James Leo C LC
Proceedings of the National Academy of Sciences of the United States of America 20180912 41
Adenovirus has enormous potential as a gene-therapy vector, but preexisting immunity limits its widespread application. What is responsible for this immune block is unclear because antibodies potently inhibit transgene expression without impeding gene transfer into target cells. Here we show that antibody prevention of adenoviral gene delivery in vivo is mediated by the cytosolic antibody receptor TRIM21. Genetic KO of TRIM21 or a single-antibody point mutation is sufficient to restore transgene ...[more]