Ontology highlight
ABSTRACT:
SUBMITTER: Ruan Y
PROVIDER: S-EPMC6187180 | biostudies-literature | 2018 Oct
REPOSITORIES: biostudies-literature
Ruan Yi Y Kao Kevin K Lefebvre Solène S Marchesi Arin A Corringer Pierre-Jean PJ Hite Richard K RK Scheuring Simon S
Proceedings of the National Academy of Sciences of the United States of America 20180904 41
<i>Gloeobacter violaceus</i> ligand-gated ion channel (GLIC), a proton-gated, cation-selective channel, is a prokaryotic homolog of the pentameric Cys-loop receptor ligand-gated ion channel family. Despite large changes in ion conductance, small conformational changes were detected in X-ray structures of detergent-solubilized GLIC at pH 4 (active/desensitized state) and pH 7 (closed state). Here, we used high-speed atomic force microscopy (HS-AFM) combined with a buffer exchange system to perfor ...[more]