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An interspecies barrier to tetraploid complementation and chimera formation.

ABSTRACT: To study development of the conceptus in xenogeneic environments, we assessed interspecies chimera formation as well as tetraploid complementation between mouse and rat. Overall contribution of donor PSC-derived cells was lower in interspecies chimeras than in intraspecies chimeras, and high donor chimerism was associated with anomalies or embryonic death. Organ to organ variation in donor chimerism was greater in interspecies chimeras than in intraspecies chimeras, suggesting species-specific affinity differences among interacting molecules necessary for organogenesis. In interspecies tetraploid complementation, embryo development was near normal until the stage of placental formation, after which no embryos survived.

SUBMITTER: Yamaguchi T

PROVIDER: S-EPMC6191448 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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An interspecies barrier to tetraploid complementation and chimera formation.

Yamaguchi Tomoyuki T Sato Hideyuki H Kobayashi Toshihiro T Kato-Itoh Megumi M Goto Teppei T Hara Hiromasa H Mizuno Naoaki N Yanagida Ayaka A Umino Ayumi A Hamanaka Sanae S Suchy Fabian F Masaki Hideki H Ota Yasunori Y Hirabayashi Masumi M Nakauchi Hiromitsu H

Scientific reports 20181016 1

To study development of the conceptus in xenogeneic environments, we assessed interspecies chimera formation as well as tetraploid complementation between mouse and rat. Overall contribution of donor PSC-derived cells was lower in interspecies chimeras than in intraspecies chimeras, and high donor chimerism was associated with anomalies or embryonic death. Organ to organ variation in donor chimerism was greater in interspecies chimeras than in intraspecies chimeras, suggesting species-specific a ...[more]

PMID: 30327488

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