Unknown

Dataset Information

0

A combined small RNA and transcriptome sequencing analysis reveal regulatory roles of miRNAs during anther development of Upland cotton carrying cytoplasmic male sterile Gossypium harknessii (D2) cytoplasm.


ABSTRACT: BACKGROUND:Cytoplasmic male sterility (CMS) in flowering plants is usually caused by incompatibility between mitochondrial and nuclear genomes, and can be restored by nuclear genes known as restorer-of-fertility (Rf). Although the CMS/Rf system is useful and convenient for economic production of commercial hybrid seed, the molecular mechanisms of CMS occurrence and fertility restoration in cotton are unclear. RESULTS:Here, a combined small RNA and transcriptome sequencing analysis was performed on floral buds at the meiosis stage in three-line hybrid cotton system, and differentially expressed microRNAs (DEMs) and their target genes were identified and further analyzed for a possible involvement in CMS and fertility restoration. Totally 10 and 30 differentially expressed miRNA-target gene pairs were identified in A-B and A-R comparison group, respectively. A putative regulatory network of CMS occurrence and fertility restoration-related miRNA-target pairs during anther development were then constructed. The RLM-RACE analysis showed that gra-miR7505b regulates a PPR gene (Gh_D05G3392) by cleaving precisely at the 643 nt and 748 nt sites. The further analysis indicated that the sequence variation in the binding regions of Gh_D05G3392 and Gh_D05G3356 may cause a lower cleavage efficiency of the PPR genes by miR7505b and miR7505 in R line, respectively, leading to the up-regulation of the PPR genes and fertility restoration. These results have established their genetic involvement in fertility restoration in the CMS-D2 system. CONCLUSION:Our combined miRNA and transcriptome analysis in three-line hybrid cotton system provides new insights into the molecular mechanisms of CMS occurrence and fertility restoration, which will contribute to further hybrid breeding in cotton.

SUBMITTER: Zhang B 

PROVIDER: S-EPMC6192183 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

A combined small RNA and transcriptome sequencing analysis reveal regulatory roles of miRNAs during anther development of Upland cotton carrying cytoplasmic male sterile Gossypium harknessii (D2) cytoplasm.

Zhang Bingbing B   Zhang Xuexian X   Liu Guoyuan G   Guo Liping L   Qi Tingxiang T   Zhang Meng M   Li Xue X   Wang Hailin H   Tang Huini H   Qiao Xiuqin X   Pei Wenfeng W   Shahzad Kashif K   Xing Chaozhu C   Zhang Jinfa J   Wu Jianyong J  

BMC plant biology 20181017 1


<h4>Background</h4>Cytoplasmic male sterility (CMS) in flowering plants is usually caused by incompatibility between mitochondrial and nuclear genomes, and can be restored by nuclear genes known as restorer-of-fertility (Rf). Although the CMS/Rf system is useful and convenient for economic production of commercial hybrid seed, the molecular mechanisms of CMS occurrence and fertility restoration in cotton are unclear.<h4>Results</h4>Here, a combined small RNA and transcriptome sequencing analysis  ...[more]

Similar Datasets

| S-EPMC3998979 | biostudies-literature
2013-10-31 | GSE51779 | GEO
| S-EPMC10071737 | biostudies-literature
2013-10-31 | E-GEOD-51779 | biostudies-arrayexpress
| S-EPMC6013946 | biostudies-literature
| S-EPMC6589528 | biostudies-literature
| S-EPMC5841646 | biostudies-literature
| PRJNA670627 | ENA
| S-EPMC8342446 | biostudies-literature
| S-EPMC10834806 | biostudies-literature