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Photo-controlled cell-specific metabolic labeling of RNA.

ABSTRACT: Elucidating gene expression programs within a cell-specific manner is a grand challenge for biologists. Harder still is the ability to have kinetic control over such experiments. Metabolic labeling with bioorthogonally-functionalized metabolic intermediates provides a means to profile RNA expression in a cell-specific manner, but there is still a lack of kinetic resolution. Herein we present the synthesis and evaluation of photocaged metabolic uracil intermediates. We compare the photo-decaging properties and demonstrate their utility in metabolic labeling experiments in a cell-specific manner. We anticipate that our approach will have far-reaching impact as it provides control over tagging of nascent RNA.

SUBMITTER: Feng C

PROVIDER: S-EPMC6193551 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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Photo-controlled cell-specific metabolic labeling of RNA.

Feng C C Li Y Y Spitale R C RC

Organic & biomolecular chemistry 20170601 24

Elucidating gene expression programs within a cell-specific manner is a grand challenge for biologists. Harder still is the ability to have kinetic control over such experiments. Metabolic labeling with bioorthogonally-functionalized metabolic intermediates provides a means to profile RNA expression in a cell-specific manner, but there is still a lack of kinetic resolution. Herein we present the synthesis and evaluation of photocaged metabolic uracil intermediates. We compare the photo-decaging ...[more]

PMID: 28569333

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