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Protected pyrimidine nucleosides for cell-specific metabolic labeling of RNA.

ABSTRACT: RNA molecules can perform a myriad of functions, from the regulation of gene expression to providing the genetic blueprint for protein synthesis. Characterizing RNA expression dynamics, in a cell-specific manner, still remains a great challenge in biology. Herein we present a new set of protected alkynyl nucleosides for cell-specific metabolic labeling of RNA. We anticipate these analogs will find wide spread utility toward the goal of understanding RNA expression in complex cellular and tissue environments, even within living animals.

SUBMITTER: Beasley S

PROVIDER: S-EPMC6483386 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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Protected pyrimidine nucleosides for cell-specific metabolic labeling of RNA.

Beasley Samantha S Nguyen Kim K Fazio Michael M Spitale Robert C RC

Tetrahedron letters 20180915 44

RNA molecules can perform a myriad of functions, from the regulation of gene expression to providing the genetic blueprint for protein synthesis. Characterizing RNA expression dynamics, in a cell-specific manner, still remains a great challenge in biology. Herein we present a new set of protected alkynyl nucleosides for cell-specific metabolic labeling of RNA. We anticipate these analogs will find wide spread utility toward the goal of understanding RNA expression in complex cellular and tissue ...[more]

PMID: 31031425

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