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Glycan binding patterns of human rotavirus P[10] VP8* protein.


ABSTRACT: BACKGROUND:Rotaviruses (RVs) are a major cause of acute children gastroenteritis. The rotavirus P [10] belongs to P[I] genogroup of group A rotaviruses that mainly infect animals, while the rotavirus P [10] was mainly identified from human infection. The rotavirus P [10] is an unusual genotype and the recognition pattern of cellular receptors remains unclear. METHODS:We expressed and purified the RV P [10] VP8* protein and investigated the saliva and oligosaccharide binding profiles of the protein. A homology model of the P [10] VP8* core protein was built and the superimposition structural analysis of P [10] VP8* protein on P [19] VP8* in complex with mucin core 2 was performed to explore the possible docking structural basis of P [10] VP8* and mucin cores. RESULTS:Our data showed that rotavirus P [10] VP8* protein bound to all ABO secretor and non-secretor saliva. The rotavirus P [10] could bind strongly to mucin core 2 and weakly to mucin core 4. The homology modeling indicated that RV P [10] VP8* binds to mucin core 2 using a potential glycan binding site that is the same to P [19] VP8* belonging to P[II] genogroup. CONCLUSION:Our results suggested an interaction of rotavirus P [10] VP8* protein with mucin core 2 and mucin core 4. These findings offer potential for elucidating the mechanism of RV A host specificity, evolution and epidemiology.

SUBMITTER: Pang LL 

PROVIDER: S-EPMC6195756 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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Glycan binding patterns of human rotavirus P[10] VP8* protein.

Pang Li-Li LL   Wang Meng-Xuan MX   Sun Xiao-Man XM   Yuan Yue Y   Qing Yu Y   Xin Yan Y   Zhang Jia-Yan JY   Li Dan-di DD   Duan Zhao-Jun ZJ  

Virology journal 20181019 1


<h4>Background</h4>Rotaviruses (RVs) are a major cause of acute children gastroenteritis. The rotavirus P [10] belongs to P[I] genogroup of group A rotaviruses that mainly infect animals, while the rotavirus P [10] was mainly identified from human infection. The rotavirus P [10] is an unusual genotype and the recognition pattern of cellular receptors remains unclear.<h4>Methods</h4>We expressed and purified the RV P [10] VP8* protein and investigated the saliva and oligosaccharide binding profil  ...[more]

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