A novel approach for fast detection of sepsis with Gram-negative bacterial infection.
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ABSTRACT:
SUBMITTER: Lin J
PROVIDER: S-EPMC6196396 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
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A novel approach for fast detection of sepsis with Gram-negative bacterial infection.
Lin Jingan J Chen Long L Lin Jiansen J Wu Haiping H Okunieff Paul P Wu Bing B Yang Bing B Chen Jinrong J Lin Jianhua J Zhang Lurong L
Microbial biotechnology 20181101 6
Sepsis, a life-threatening systemic infection, requires quick treatment. Gram-negative bacteria (GNB) are the major causative pathogens and their endotoxin can be a surrogate biomarker for diagnosis. We explored a fast identification of GNB by first culturing blood to increase endotoxin levels and then detecting endotoxin by Tachypleus amebocyte lysate (TAL) with kinetic turbidimetric assay (KT-TAL). Heating samples could significantly increase the endotoxin released from GNB; speed and time of ...[more]
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