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Data on whole genome shotgun sequencing report of clinical S. maltophilia strains from India.

ABSTRACT: Stenotrophomonas maltophilia is an important emerging nosocomial pathogen with broad level multi-drug resistance. There is a lack of genomic information on S. maltophilia to understand the antimicrobial resistance (AMR) mechanism behind. The data article reports on whole genome sequence information of 9 clinical S. maltophilia strains isolated from a tertiary care hospital in India. Isolates were sequenced using Ion Torrent PGM platform. Raw reads were assembled and annotated, where the genome size ranged from ~?3.2 to ~?4.5?Mb with average 57.6× coverage. AMR genes blaL1, blaL2, Smqnr, aac(6?)-lz and aph(3?)-llc were observed among the isolates in addition to multiple virulence factors. Five isolates were identified to be ST15, ST283, ST284, ST285 and ST286.

SUBMITTER: Devanga Ragupathi NK

PROVIDER: S-EPMC6197317 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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Data on whole genome shotgun sequencing report of clinical S. maltophilia strains from India.

Devanga Ragupathi Naveen Kumar NK Veeraraghavan Balaji B

Data in brief 20181004

Stenotrophomonas maltophilia is an important emerging nosocomial pathogen with broad level multi-drug resistance. There is a lack of genomic information on S. maltophilia to understand the antimicrobial resistance (AMR) mechanism behind. The data article reports on whole genome sequence information of 9 clinical S. maltophilia strains isolated from a tertiary care hospital in India. Isolates were sequenced using Ion Torrent PGM platform. Raw reads were assembled and annotate ...[more]

PMID: 30364539

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Project description:Microorganisms are ubiquitous in the biosphere, playing a crucial role in both biogeochemistry of the planet and human health. However, identifying these microorganisms and defining their function are challenging. Widely used approaches in comparative metagenomics, 16S amplicon sequencing and whole genome shotgun sequencing (WGS), have provided access to DNA sequencing analysis to identify microorganisms and evaluate diversity and abundance in various environments. However, advances in parallel high-throughput DNA sequencing in the past decade have introduced major hurdles, namely standardization of methods, data storage, reproducible interoperability of results, and data sharing. The National Ecological Observatory Network (NEON), established by the National Science Foundation, enables all researchers to address queries on a regional to continental scale around a variety of environmental challenges and provide high-quality, integrated, and standardized data from field sites across the U.S. As the amount of metagenomic data continues to grow, standardized procedures that allow results across projects to be assessed and compared is becoming increasingly important in the field of metagenomics. We demonstrate the feasibility of using publicly available NEON soil metagenomic sequencing datasets in combination with open access Metagenomics Rapid Annotation using the Subsystem Technology (MG-RAST) server to illustrate advantages of WGS compared to 16S amplicon sequencing. Four WGS and four 16S amplicon sequence datasets, from surface soil samples prepared by NEON investigators, were selected for comparison, using standardized protocols collected at the same locations in Colorado between April-July 2014. The dominant bacterial phyla detected across samples agreed between sequencing methodologies. However, WGS yielded greater microbial resolution, increased accuracy, and allowed identification of more genera of bacteria, archaea, viruses, and eukaryota, and putative functional genes that would have gone undetected using 16S amplicon sequencing. NEON open data will be useful for future studies characterizing and quantifying complex ecological processes associated with changing aquatic and terrestrial ecosystems.

Dataset Information

Data on whole genome shotgun sequencing report of clinical S. maltophilia strains from India.

Publications

Data on whole genome shotgun sequencing report of clinical <i>S. maltophilia</i> strains from India.

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