Dataset Information

Impact of a single HLA-A24:02-associated escape mutation on the detrimental effect of HLA-B35:01 in HIV-1 control.

ABSTRACT:

Background

HLA-B*35 is an HLA allele associated with rapid progression to AIDS. However, a mechanism underlying the detrimental effect of HLA-B*35 on disease outcome remains unknown. Recent studies demonstrated that most prevalent subtype HLA-B*35:01 is a detrimental allele in HIV-1 clade B-infected individuals. We here investigated the effect of mutations within the epitopes on HLA-B*35:01-restricted CD8⁺ T cells having abilities to suppress HIV-1 replication.

Methods

We analyzed 16 HLA-B*35:01-restricted epitope-specific T cells in 63 HIV-1 clade B-infected Japanese B*35:01⁺ individuals and identified HLA-B*35:01-restricted CD8⁺ T cells having abilities to suppress HIV-1 replication. We further analyzed the effect of HLA-associated mutations on the ability of these T cells.

Findings

The breadth of T cell responses to 4 epitopes was inversely associated with plasma viral load (pVL). However, the accumulation of an Y135F mutation in NefYF9 out of the 4 epitopes, which is selected by HLA-A*24:02-restricted T cells, affected the ability of YF9-specific T cells to suppress HIV-1 replication. HLA-B*35:01⁺ individuals harboring this mutation had much higher pVL than those without it. YF9-specific T cells failed to suppress replication of the Y135F mutant in vitro. These results indicate that this mutation impairs suppression of HIV-1 replication by YF9-specific T cells.

Interpretation

These findings indicate that the Y135F mutation is a key factor underlying the detrimental effect of HLA-B*35:01 on disease outcomes in HIV-1 clade B-infected individuals. FUND: Grants-in-aid for AIDS Research from AMED and for scientific research from the Ministry of Education, Science, Sports, and Culture, Japan.

SUBMITTER: Murakoshi H

PROVIDER: S-EPMC6197679 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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Publications

Impact of a single HLA-A24:02-associated escape mutation on the detrimental effect of HLA-B35:01 in HIV-1 control.

Murakoshi Hayato H Koyanagi Madoka M Akahoshi Tomohiro T Chikata Takayuki T Kuse Nozomi N Gatanaga Hiroyuki H Rowland-Jones Sarah L SL Oka Shinichi S Takiguchi Masafumi M

EBioMedicine 20180922

<h4>Background</h4>HLA-B*35 is an HLA allele associated with rapid progression to AIDS. However, a mechanism underlying the detrimental effect of HLA-B*35 on disease outcome remains unknown. Recent studies demonstrated that most prevalent subtype HLA-B*35:01 is a detrimental allele in HIV-1 clade B-infected individuals. We here investigated the effect of mutations within the epitopes on HLA-B*35:01-restricted CD8<sup>+</sup> T cells having abilities to suppress HIV-1 replication.<h4>Methods</h4> ...[more]

PMID: 30249546

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Dataset Information

Impact of a single HLA-A*24:02-associated escape mutation on the detrimental effect of HLA-B*35:01 in HIV-1 control.

Background

Methods

Findings

Interpretation

Publications

Impact of a single HLA-A*24:02-associated escape mutation on the detrimental effect of HLA-B*35:01 in HIV-1 control.

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Impact of a single HLA-A24:02-associated escape mutation on the detrimental effect of HLA-B35:01 in HIV-1 control.

Impact of a single HLA-A24:02-associated escape mutation on the detrimental effect of HLA-B35:01 in HIV-1 control.