Dataset Information

Simple and cost-effective laboratory methods to evaluate and validate cell-free DNA isolation.

ABSTRACT:

Objective

In the present study, we investigated different simple and cost effective methods to evaluate and validate cell free DNA (cfDNA) isolation. The ability of the QIAamp DNA Blood Mini Kit method to extract cfDNA was assessed by several approaches, including purification of endogenous cfDNA and exogenous spike-in control material, prior to plasma extraction, and followed by quantitative-PCR.

Results

Using QIAamp DNA Blood Mini kit, nearly 27% (380 bp) to 35% (173 bp) cfDNA was recovered with a higher recovery of smaller size cfDNA (173 bp) in comparison to larger ones (380 bp). These simple laboratory methods can be used to assess the efficiency of any cfDNA isolation method.

SUBMITTER: Mojtabanezhad Shariatpanahi A

PROVIDER: S-EPMC6199704 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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Publications

Simple and cost-effective laboratory methods to evaluate and validate cell-free DNA isolation.

Mojtabanezhad Shariatpanahi Afsaneh A Rokni Parisa P Shahabi Elaheh E Varshoee Tabrizi Fatemeh F Kerachian Mohammad Amin MA

BMC research notes 20181023 1

<h4>Objective</h4>In the present study, we investigated different simple and cost effective methods to evaluate and validate cell free DNA (cfDNA) isolation. The ability of the QIAamp DNA Blood Mini Kit method to extract cfDNA was assessed by several approaches, including purification of endogenous cfDNA and exogenous spike-in control material, prior to plasma extraction, and followed by quantitative-PCR.<h4>Results</h4>Using QIAamp DNA Blood Mini kit, nearly 27% (380 bp) to 35% (173 bp) cfDNA w ...[more]

PMID: 30352614

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Dataset Information

Simple and cost-effective laboratory methods to evaluate and validate cell-free DNA isolation.

Objective

Results

Publications

Simple and cost-effective laboratory methods to evaluate and validate cell-free DNA isolation.

Similar Datasets

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