Ontology highlight
ABSTRACT:
SUBMITTER: Chatterjee P
PROVIDER: S-EPMC6200363 | biostudies-literature | 2018 Oct
REPOSITORIES: biostudies-literature
Chatterjee Pranam P Jakimo Noah N Jacobson Joseph M JM
Science advances 20181024 10
RNA-guided DNA endonucleases of the CRISPR-Cas system are widely used for genome engineering and thus have numerous applications in a wide variety of fields. CRISPR endonucleases, however, require a specific protospacer adjacent motif (PAM) flanking the target site, thus constraining their targetable sequence space. In this study, we demonstrate the natural PAM plasticity of a highly similar, yet previously uncharacterized, Cas9 from <i>Streptococcus canis</i> (ScCas9) through rational manipulat ...[more]