Ontology highlight
ABSTRACT:
SUBMITTER: Zhang Q
PROVIDER: S-EPMC8643646 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
Zhang Qian Q Chen Ziting Z Wang Fangzhu F Zhang Siqi S Chen Hongyu H Gu Xueying X Wen Fengcai F Jin Jiachuan J Zhang Xia X Huang Xingxu X Shen Bin B Sun Bo B
Nucleic acids research 20211201 21
Streptococcus pyogenes Cas9 (SpCas9), a programmable RNA-guided DNA endonuclease, has been widely repurposed for biological and medical applications. Critical interactions between SpCas9 and DNA confer the high specificity of the enzyme in genome engineering. Here, we unveil that an essential SpCas9-DNA interaction located beyond the protospacer adjacent motif (PAM) is realized through electrostatic forces between four positively charged lysines among SpCas9 residues 1151-1156 and the negatively ...[more]