Ontology highlight
ABSTRACT:
SUBMITTER: Chada N
PROVIDER: S-EPMC6200364 | biostudies-literature | 2018 Oct
REPOSITORIES: biostudies-literature
Science advances 20181024 10
SecA is the critical adenosine triphosphatase that drives preprotein transport through the translocon, SecYEG, in <i>Escherichia coli</i>. This process is thought to be regulated by conformational changes of specific domains of SecA, but real-time, real-space measurement of these changes is lacking. We use single-molecule atomic force microscopy (AFM) to visualize nucleotide-dependent conformations and conformational dynamics of SecA. Distinct topographical populations were observed in the prese ...[more]