Ontology highlight
ABSTRACT:
SUBMITTER: Chu T
PROVIDER: S-EPMC6204104 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Chu Tinyi T Rice Edward J EJ Booth Gregory T GT Salamanca H Hans HH Wang Zhong Z Core Leighton J LJ Longo Sharon L SL Corona Robert J RJ Chin Lawrence S LS Lis John T JT Kwak Hojoong H Danko Charles G CG
Nature genetics 20181022 11
The human genome encodes a variety of poorly understood RNA species that remain challenging to identify using existing genomic tools. We developed chromatin run-on and sequencing (ChRO-seq) to map the location of RNA polymerase for almost any input sample, including samples with degraded RNA that are intractable to RNA sequencing. We used ChRO-seq to map nascent transcription in primary human glioblastoma (GBM) brain tumors. Enhancers identified in primary GBMs resemble open chromatin in the nor ...[more]