Unknown

Dataset Information

0

Novel solid-phase strategy for the synthesis of ligand-targeted fluorescent-labelled chelating peptide conjugates as a theranostic tool for cancer.


ABSTRACT: In this article, we have successfully designed and demonstrated a novel continuous process for assembling targeting ligands, peptidic spacers, fluorescent tags and a chelating core for the attachment of cytotoxic molecules, radiotracers, nanomaterials in a standard Fmoc solid-phase peptide synthesis in high yield and purity. The differentially protected Fmoc-Lys-(Tfa)-OH plays a vital role in attaching fluorescent tags while growing the peptide chain in an uninterrupted manner. The methodology is versatile for solid-phase resins that are sensitive to mild and strong acidic conditions when acid-sensitive side chain amino protecting groups such as Trt (chlorotrityl), Mtt (4-methyltrityl), Mmt (4-methoxytrityl) are employed to synthesise the ligand targeted fluorescent tagged bioconjugates. Using this methodology, DUPA rhodamine B conjugate (DUPA = 2-[3-(1,3-dicarboxypropyl)ureido]pentanedioic acid), targeting prostate specific membrane antigen (PSMA) expressed on prostate, breast, bladder and brain cancers and pteroate rhodamine B, targeting folate receptor positive cancers such as ovarian, lung, endometrium as well as inflammatory diseases have been synthesized. In vitro studies using LNCaP (PSMA +ve), PC-3 (PSMA -ve, FR -ve) and CHO-? (FR +ve) cell lines and their respective competition experiments demonstrate the specificity of the newly synthesized bioconstructs for future application in fluorescent guided intra-operative imaging.

SUBMITTER: Sengupta S 

PROVIDER: S-EPMC6204756 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

altmetric image

Publications

Novel solid-phase strategy for the synthesis of ligand-targeted fluorescent-labelled chelating peptide conjugates as a theranostic tool for cancer.

Sengupta Sagnik S   Krishnan Mena Asha MA   Dudhe Premansh P   Reddy Ramesh B RB   Giri Bishnubasu B   Chattopadhyay Sudeshna S   Chelvam Venkatesh V  

Beilstein journal of organic chemistry 20181018


In this article, we have successfully designed and demonstrated a novel continuous process for assembling targeting ligands, peptidic spacers, fluorescent tags and a chelating core for the attachment of cytotoxic molecules, radiotracers, nanomaterials in a standard Fmoc solid-phase peptide synthesis in high yield and purity. The differentially protected Fmoc-Lys-(Tfa)-OH plays a vital role in attaching fluorescent tags while growing the peptide chain in an uninterrupted manner. The methodology i  ...[more]

Similar Datasets

| S-EPMC7050787 | biostudies-literature
| S-EPMC6324731 | biostudies-literature
| S-EPMC5811147 | biostudies-literature
| S-EPMC9266369 | biostudies-literature
| S-EPMC6648899 | biostudies-literature
| S-EPMC8419038 | biostudies-literature
| S-EPMC5947674 | biostudies-literature
| S-EPMC3423536 | biostudies-literature
| S-EPMC6941177 | biostudies-literature
| S-EPMC5095156 | biostudies-literature