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Rapid Fluorescent-Based Detection of New Delhi Metallo-?-Lactamases by Photo-Cross-Linking Using Conjugates of Azidonaphthalimide and Zinc(II)-Chelating Motifs.

ABSTRACT: A method for rapid detection of metallo-?-lactamases NDM-5 and NDM-7 using conjugates of azidonaphthalimide and Zn(II) chelating motifs (like sulfonamides, hydroxamate, and terpyridine) is described. Incubation and irradiation, followed by gel electrophoresis, clearly show the presence of NDMs. The o-sulfonamide-based probe has the highest efficiency of detection for both the NDMs. The proteins are detectable at nM concentrations, and the method is also selective, works both in vitro and in vivo, as revealed by cellular imaging and also with clinical isolates.

SUBMITTER: Singha M

PROVIDER: S-EPMC6648899 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Rapid Fluorescent-Based Detection of New Delhi Metallo-β-Lactamases by Photo-Cross-Linking Using Conjugates of Azidonaphthalimide and Zinc(II)-Chelating Motifs.

Singha Monisha M Kumar Gaurav G Jain Diamond D Kumar N Ganesh G Ray Debashis D Ghosh Anindya S AS Basak Amit A

ACS omega 20190621 6

A method for rapid detection of metallo-β-lactamases NDM-5 and NDM-7 using conjugates of azidonaphthalimide and Zn(II) chelating motifs (like sulfonamides, hydroxamate, and terpyridine) is described. Incubation and irradiation, followed by gel electrophoresis, clearly show the presence of NDMs. The <i>o</i>-sulfonamide-based probe has the highest efficiency of detection for both the NDMs. The proteins are detectable at nM concentrations, and the method is also selective, works both in vitro and ...[more]

PMID: 31460186

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Pterostilbene restores carbapenem susceptibility in New Delhi metallo-?-lactamase-producing isolates by inhibiting the activity of New Delhi metallo-?-lactamases.

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