Blood handling and leukocyte isolation methods impact the global transcriptome of immune cells.

ABSTRACT:

Background

Transcriptional profiling with ultra-low input methods can yield valuable insights into disease, particularly when applied to the study of immune cells using RNA-sequencing. The advent of these methods has allowed for their use in profiling cells collected in clinical trials and other studies that involve the coordination of human-derived material. To date, few studies have sought to quantify what effects that collection and handling of this material can have on resulting data.

Results

We characterized the global effects of blood handling, methods for leukocyte isolation, and preservation media on low numbers of immune cells isolated from blood. We found overall that storage/shipping temperature of blood prior to leukocyte isolation and sorting led to global chang

SUBMITTER: Goods BA 

PROVIDER: S-EPMC6208098 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature