Dataset Information

H19 Is Expressed in Hybrid Hepatocyte Nuclear Factor 4?⁺ Periportal Hepatocytes but Not Cytokeratin 19⁺ Cholangiocytes in Cholestatic Livers.

ABSTRACT: Long noncoding RNA (lncRNA) H19 is abundantly expressed in fetal liver. Its expression is significantly diminished in adult healthy liver but is re-induced in chronic liver diseases, including cholestasis. In this study, we developed a new method with combined in situ hybridization (ISH) and immunofluorescence (IF) colabeling to establish an H19 expression profile with both parenchymal and nonparenchymal cell-specific markers in the livers of cholestatic mouse models and patients with cholestasis. H19RNA⁺ cells showed no colocalization with biliary epithelial cell marker cytokeratin 19 (CK19)⁺ cholangiocytes but were immediately adjacent to biliary structures in bile duct ligation (BDL), 3,5-diethoxycarbony1-1,4-dihydrocollidine (DDC), and multidrug-resistant gene 2 knockout ( Mdr2 ^-/- ) mouse models and in human primary biliary cholangitis (PBC) and primary sclerosing cholangitis (PSC) liver specimens. In contrast, double-positive H19RNA⁺/sex-determining region Y (SRY)-box 9 (SOX9)⁺ ductal progenitor cells, H19RNA⁺/hepatocyte nuclear factor 4? (HNF4?)⁺ hepatocytes, H19RNA⁺/F4/80⁺ Kupffer cells, HNF4?⁺/SOX9⁺ hybrid hepatocytes, as well as triple-positive H19 RNA⁺/HNF4?⁺/SOX9⁺ periportal hepatocytes were identified. In addition, H19 RNA could not be detected in mesenchymal cell marker desmin⁺ cells. Furthermore, H19 RNA was predominately detected in cytoplasm with a small amount at the interspace with neighboring cells. Conclusion: H19RNA is localized in HNF4?⁺ periportal hepatocytes, SOX9⁺ ductal progenitor cells, and F4/80⁺ Kupffer cells but not in CK19⁺ cholangiocytes and desmin⁺ stellate cells in cholestatic livers.

SUBMITTER: Jiang Y

PROVIDER: S-EPMC6211330 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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H19 Is Expressed in Hybrid Hepatocyte Nuclear Factor 4α+ Periportal Hepatocytes but Not Cytokeratin 19+ Cholangiocytes in Cholestatic Livers.

Jiang YanChao Y Huang Yi Y Cai ShiYing S Song YongFeng Y Boyer James L JL Zhang KeZhong K Gao Ling L Zhao JiaJun J Huang WenDong W Liang Guang G Liangpunsakul Suthat S Wang Li L

Hepatology communications 20180924 11

Long noncoding RNA (lncRNA) H19 is abundantly expressed in fetal liver. Its expression is significantly diminished in adult healthy liver but is re-induced in chronic liver diseases, including cholestasis. In this study, we developed a new method with combined in situ hybridization (ISH) and immunofluorescence (IF) colabeling to establish an H19 expression profile with both parenchymal and nonparenchymal cell-specific markers in the livers of cholestatic mouse models and patients w ...[more]

PMID: 30411082

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Dataset Information

H19 Is Expressed in Hybrid Hepatocyte Nuclear Factor 4?⁺ Periportal Hepatocytes but Not Cytokeratin 19⁺ Cholangiocytes in Cholestatic Livers.

Publications

H19 Is Expressed in Hybrid Hepatocyte Nuclear Factor 4α<sup>+</sup> Periportal Hepatocytes but Not Cytokeratin 19<sup>+</sup> Cholangiocytes in Cholestatic Livers.

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Dataset Information

H19 Is Expressed in Hybrid Hepatocyte Nuclear Factor 4?+ Periportal Hepatocytes but Not Cytokeratin 19+ Cholangiocytes in Cholestatic Livers.

Publications

H19 Is Expressed in Hybrid Hepatocyte Nuclear Factor 4α<sup>+</sup> Periportal Hepatocytes but Not Cytokeratin 19<sup>+</sup> Cholangiocytes in Cholestatic Livers.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

H19 Is Expressed in Hybrid Hepatocyte Nuclear Factor 4?⁺ Periportal Hepatocytes but Not Cytokeratin 19⁺ Cholangiocytes in Cholestatic Livers.