Ontology highlight
ABSTRACT:
SUBMITTER: Kalita CA
PROVIDER: S-EPMC6211638 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Kalita Cynthia A CA Brown Christopher D CD Freiman Andrew A Isherwood Jenna J Wen Xiaoquan X Pique-Regi Roger R Luca Francesca F
Genome research 20180925 11
Many variants associated with complex traits are in noncoding regions and contribute to phenotypes by disrupting regulatory sequences. To characterize these variants, we developed a streamlined protocol for a high-throughput reporter assay, Biallelic Targeted STARR-seq (BiT-STARR-seq), that identifies allele-specific expression (ASE) while accounting for PCR duplicates through unique molecular identifiers. We tested 75,501 oligos (43,500 SNPs) and identified 2720 SNPs with significant ASE (FDR < ...[more]