Ontology highlight
ABSTRACT:
SUBMITTER: Hauschild KE
PROVIDER: S-EPMC2760306 | biostudies-literature | 2009 Jul
REPOSITORIES: biostudies-literature
Bioorganic & medicinal chemistry letters 20090503 14
Determining the sequence specifity of DNA binding molecules is a non-trivial task. Here we describe the development of a platform for assaying the sequence specificity of DNA ligands using label free detection on high density DNA microarrays. This is achieved by combining Cognate Site Identification (CSI) with Fluorescence Intercalation Displacement (FID) to create CSI-FID. We use the well-studied small molecule DNA ligand netropsin to develop this high throughput platform. Analysis of the DNA b ...[more]