Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Microsphere-based interferometric optical probe.

ABSTRACT: Fluorescent optical probes have rapidly transformed our understanding of complex biological systems by providing specific information on biological targets in the natural living state. However, their utility is often limited by insufficient brightness, photostability, and multiplexing capacity. Here, we report a conceptually new optical probe, termed 'reflectophore', which is based on the spectral interference from a dielectric microsphere. Reflectophores are orders-of-magnitudes brighter than conventional fluorophores and are free from photobleaching, enabling practically unlimited readout at high fidelity. They also offer high-degree multiplexing, encoded in their optical size, which can be readily decoded through interferometric detection with nanoscale accuracy, even in turbid biological media. Furthermore, we showcase their biological applications in cellular barcoding and microenvironmental sensing of a target protein and local electric field.

SUBMITTER: Jo Y

PROVIDER: S-EPMC6212483 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Publications

Microsphere-based interferometric optical probe.

Jo Yongjae Y Kwon Junhwan J Kim Moonseok M Choi Wonshik W Choi Myunghwan M

Nature communications 20181101 1

Fluorescent optical probes have rapidly transformed our understanding of complex biological systems by providing specific information on biological targets in the natural living state. However, their utility is often limited by insufficient brightness, photostability, and multiplexing capacity. Here, we report a conceptually new optical probe, termed 'reflectophore', which is based on the spectral interference from a dielectric microsphere. Reflectophores are orders-of-magnitudes brighter than c ...[more]

PMID: 30385746

Similar Datasets

A suspended graphene-based optical interferometric surface stress sensor for selective biomolecular detection.

Project description:Graphene-based sensors are of great interest in research due to their high specific surface area and high electron mobility that make them suitable for numerous advanced applications. In this paper, selective molecular detection using an antigen-antibody reaction on suspended graphene with a cavity-sealing structure was demonstrated. The suspended graphene sealed nanocavities in a pre-patterned Si substrate, which increased robustness and allowed the use of wet chemical processes for surface functionalization of the suspended graphene to achieve selective molecular binding. The selectivity was evaluated by nanomechanical deflection induced by molecular adsorption on the suspended graphene, resulting in spectral shifts in the optical interference between the suspended graphene and Si substrate. The chemically functionalized suspended graphene enables the analysis of intermolecular interactions and molecular kinetics by colorimetry using optical interference.

| S-EPMC9417660 | biostudies-literature

Real-time in vivo computed optical interferometric tomography.

Project description:High-resolution real-time tomography of scattering tissues is important for many areas of medicine and biology1-6. However, the compromise between transverse resolution and depth-of-field in addition to low sensitivity deep in tissue continue to impede progress towards cellular-level volumetric tomography. Computed imaging has the potential to solve these long-standing limitations. Interferometric synthetic aperture microscopy (ISAM)7-9 is a computed imaging technique enabling high-resolution volumetric tomography with spatially invariant resolution. However, its potential for clinical diagnostics remains largely untapped since full volume reconstructions required lengthy postprocessing, and the phase-stability requirements have been difficult to satisfy in vivo. Here we demonstrate how 3-D Fourier-domain resampling, in combination with high-speed optical coherence tomography (OCT), can achieve high-resolution in vivo tomography. Enhanced depth sensitivity was achieved over a depth-of-field extended in real time by more than an order of magnitude. This work lays the foundation for high-speed volumetric cellular-level tomography.

| S-EPMC3742112 | biostudies-literature

High-speed optical coherence tomography by circular interferometric ranging.

Project description:Existing three-dimensional optical imaging methods excel in controlled environments but are difficult to deploy over large, irregular and dynamic fields. This has limited imaging in areas such as material inspection and medicine. To better address these applications, we developed methods in optical coherence tomography (OCT) to efficiently interrogate sparse scattering fields, i.e., those in which most locations (voxels) do not generate meaningful signal. Frequency comb sources are used to superimpose reflected signals from equispaced locations through optical subsampling. This results in circular ranging, and reduces the number of measurements required to interrogate large volumetric fields. As a result, signal acquisition barriers that have limited speed and field in OCT are avoided. With a new ultrafast, time-stretched frequency comb laser design operating with 7.6 MHz to 18.9 MHz repetition rates, we achieved imaging of multi-cm3 fields at up to 7.5 volumes per second.

| S-EPMC5894866 | biostudies-literature

Interferometric control of the absorption in optical patch antennas.

Project description:Optical patch nano-antennas possess unique absorption, field enhancement and concentration capabilities - but their crosssection, as well as their response outside of normal incidence are not well understood. Here we explain the large cross-section by considering that each patch nanoantenna is a cavity excited from both sides. Such a simple physical picture allows to fully understand the influence of the angle of incidence - that odd resonances have a very high absorption cross-section which decreases when the incidence angle increases, while even resonances cannot be excited in normal incidence. A direct application would be to use these structures as an optical nanometric set-square.

| S-EPMC5462803 | biostudies-literature

Indentation probe with optical fibre array-based optical coherence tomography for material deformation.

Project description:We present a new optomechanical probe for mechanical testing of soft matter. The probe consists of a micromachined cantilever equipped with an indenting sphere, and an array of 16 single-mode optical fibres, which are connected to an optical coherence tomography (OCT) system that allows subsurface analysis of the sample during the indentation stroke. To test our device and its capability, we performed indentation on a PDMS-based phantom. Our findings demonstrate that Common Path (CP)-OCT via lensed optical fibres can be successfully combined with a microindentation sensor to visualise the phantom's deformation profile at different indentation depths and locations in real time. LAY DESCRIPTION: This work presents a new approach to simultaneously perform micro-indentation experiments and OCT imaging. An optical fiber array-based sensor is used to develop a new hybrid tool where micro-indentation is combined with optical coherence tomography. The sensor is therefore capable of compressing a sample with a small force and simultaneously collecting OCT depth profiles underneath and around the indentation point. This method offers the opportunity to characterize the mechanical properties of soft materials and simultaneously visualize their deformation profile. The ability to integrate OCT imaging with indentation technology is promising for the non-invasive and precise characterization of different soft materials.

| S-EPMC8248032 | biostudies-literature

Dual-Angle Interferometric Scattering Microscopy for Optical Multiparametric Particle Characterization.

Project description:Traditional single-nanoparticle sizing using optical microscopy techniques assesses size via the diffusion constant, which requires suspended particles to be in a medium of known viscosity. However, these assumptions are typically not fulfilled in complex natural sample environments. Here, we introduce dual-angle interferometric scattering microscopy (DAISY), enabling optical quantification of both size and polarizability of individual nanoparticles (radius <170 nm) without requiring a priori information regarding the surrounding media or super-resolution imaging. DAISY achieves this by combining the information contained in concurrently measured forward and backward scattering images through twilight off-axis holography and interferometric scattering (iSCAT). Going beyond particle size and polarizability, single-particle morphology can be deduced from the fact that the hydrodynamic radius relates to the outer particle radius, while the scattering-based size estimate depends on the internal mass distribution of the particles. We demonstrate this by differentiating biomolecular fractal aggregates from spherical particles in fetal bovine serum at the single-particle level.

| S-EPMC10870763 | biostudies-literature

Broadband Optical Activity Spectroscopy with Interferometric Fourier-Transform Balanced Detection.

Project description:Spectrally resolved measurements of optical activity, such as circular dichroism (CD) and optical rotatory dispersion (ORD), are powerful tools to study chiroptical properties of (bio)molecular and nanoplasmonic systems. The wider utilization of these techniques, however, has been impeded by the bulky and slow design of conventional spectropolarimeters, which have been limited to a narrowband scanning approach for more than 50 years. In this work, we demonstrate broadband measurements of optical activity by combining a balanced detection scheme with interferometric Fourier-transform spectroscopy. The setup utilizes a linearly polarized light field that creates an orthogonally polarized weak chiral free-induction-decay field, along with a phase-locked achiral transmitted signal, which serves as the local oscillator for heterodyne amplification. By scanning the delay between the two fields with a birefringent common-path interferometer and recording their interferogram with a balanced detector that measures polarization rotation, broadband CD and ORD spectra are retrieved simultaneously with a Fourier transform. Using an incoherent thermal light source, we achieve state-of-the-art sensitivity for CD and ORD across a broad wavelength range in a remarkably simple setup. We further demonstrate the potential of our technique for highly sensitive measurements of glucose concentration and the real-time monitoring of ground-state chemical reactions. The setup also accepts broadband pulses and will be suitable for broadband transient optical activity spectroscopy and broadband optical activity imaging.

| S-EPMC8377715 | biostudies-literature

Interferometric near-infrared spectroscopy directly quantifies optical field dynamics in turbid media.

Project description:Sensing and imaging methods based on the dynamic scattering of coherent light (including laser speckle, laser Doppler, diffuse correlation spectroscopy, dynamic light scattering, and diffusing wave spectroscopy) quantify scatterer motion using light intensity fluctuations. The underlying optical field autocorrelation, rather than being measured directly, is typically inferred from the intensity autocorrelation through the Siegert relationship, assuming that the scattered field obeys Gaussian statistics. Here, we demonstrate interferometric near-infrared spectroscopy for measuring the time-of-flight (TOF) resolved field and intensity autocorrelations in turbid media. We find that the Siegert relationship breaks down for short TOFs due to static paths whose optical field does not decorrelate over experimental time scales. We also show that eliminating such paths by polarization gating restores the validity of the Siegert relationship. The unique capability of measuring optical field autocorrelations, as demonstrated here, enables the study of non-Gaussian and non-ergodic light scattering processes. Moreover, direct measurements of field autocorrelations are more efficient than indirect measurements based on intensity autocorrelations. Thus, optical field measurements may improve the quantiffcation of scatterer dynamics with coherent light.

| S-EPMC6205232 | biostudies-literature

Spectral interferometric microscopy reveals absorption by individual optical nanoantennas from extinction phase.

Project description:Optical antennas transform light from freely propagating waves into highly localized excitations that interact strongly with matter. Unlike their radio frequency counterparts, optical antennas are nanoscopic and high frequency, making amplitude and phase measurements challenging and leaving some information hidden. Here we report a novel spectral interferometric microscopy technique to expose the amplitude and phase response of individual optical antennas across an octave of the visible to near-infrared spectrum. Although it is a far-field technique, we show that knowledge of the extinction phase allows quantitative estimation of nanoantenna absorption, which is a near-field quantity. To verify our method we characterize gold ring-disk dimers exhibiting Fano interference. Our results reveal that Fano interference only cancels a bright mode's scattering, leaving residual extinction dominated by absorption. Spectral interference microscopy has the potential for real-time and single-shot phase and amplitude investigations of isolated quantum and classical antennas with applications across the physical and life sciences.

| S-EPMC4015323 | biostudies-literature

Photoacoustic Mouse Brain Imaging Using an Optical Fabry-Perot Interferometric Ultrasound Sensor.

Project description:Photoacoustic (PA, or optoacoustic, OA) mesoscopy is a powerful tool for mouse cerebral imaging, which offers high resolution three-dimensional (3D) images with optical absorption contrast inside the optically turbid brain. The image quality of a PA mesoscope relies on the ultrasonic transducer which detects the PA signals. An all-optical ultrasound sensor based on a Fabry-Pérot (FP) polymer cavity has the following advantages: broadband frequency response, wide angular coverage and small footprint. Here, we present 3D PA mesoscope for mouse brain imaging using such an optical sensor. A heating laser was used to stabilize the sensor's cavity length during the imaging process. To acquire data for a 3D angiogram of the mouse brain, the sensor was mounted on a translation stage and raster scanned. 3D images of the mouse brain vasculature were reconstructed which showed cerebrovascular structure up to a depth of 8 mm with high quality. Imaging segmentation and dual wavelength imaging were performed to demonstrate the potential of the system in preclinical brain research.

| S-EPMC8165253 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data