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Dual-Angle Interferometric Scattering Microscopy for Optical Multiparametric Particle Characterization.


ABSTRACT: Traditional single-nanoparticle sizing using optical microscopy techniques assesses size via the diffusion constant, which requires suspended particles to be in a medium of known viscosity. However, these assumptions are typically not fulfilled in complex natural sample environments. Here, we introduce dual-angle interferometric scattering microscopy (DAISY), enabling optical quantification of both size and polarizability of individual nanoparticles (radius <170 nm) without requiring a priori information regarding the surrounding media or super-resolution imaging. DAISY achieves this by combining the information contained in concurrently measured forward and backward scattering images through twilight off-axis holography and interferometric scattering (iSCAT). Going beyond particle size and polarizability, single-particle morphology can be deduced from the fact that the hydrodynamic radius relates to the outer particle radius, while the scattering-based size estimate depends on the internal mass distribution of the particles. We demonstrate this by differentiating biomolecular fractal aggregates from spherical particles in fetal bovine serum at the single-particle level.

SUBMITTER: Olsen E 

PROVIDER: S-EPMC10870763 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature

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Dual-Angle Interferometric Scattering Microscopy for Optical Multiparametric Particle Characterization.

Olsén Erik E   García Rodríguez Berenice B   Skärberg Fredrik F   Parkkila Petteri P   Volpe Giovanni G   Höök Fredrik F   Sundås Midtvedt Daniel D  

Nano letters 20240131 6


Traditional single-nanoparticle sizing using optical microscopy techniques assesses size via the diffusion constant, which requires suspended particles to be in a medium of known viscosity. However, these assumptions are typically not fulfilled in complex natural sample environments. Here, we introduce dual-angle interferometric scattering microscopy (DAISY), enabling optical quantification of both size and polarizability of individual nanoparticles (radius <170 nm) without requiring <i>a priori  ...[more]

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