RNAi-mediated silencing of spermidine synthase gene results in reduced reproductive potential in tobacco.

ABSTRACT: Spermidine belongs to a class of polycationic compounds known as polyamines. Polyamines are known to be involved in a wide range of biological processes but the exact role and contribution of different polyamines to these processes are still not clear. In the present study, we have tried to understand the contribution of triamine spermidine to the growth and development of tobacco by downregulating spermidine synthase gene (SPDS) using RNA interference. Down-regulatioin of SPDS gene resulted in decreased spermidine levels and a slight increase in the levels of its precursor, the diamine putrescine and the molecule downstream of Spd, the tetraamine spermine. While the vegetative growth of the transgenics remained largely unaffected, SPDS down-regulation resulted in smal

SUBMITTER: Choubey A 

PROVIDER: S-EPMC6214437 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature